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You can make changes to images in Photoshop using _Adjustment layers_ — layers that control the transparency of a particular part of the image or group of layers. You use these layers to reduce or increase the transparency of parts of an image. You can then manipulate the image layer by layer, or you can change an entire group of layers at once.

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Making Your Own Keyboard Shortcuts for Photoshop Elements

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How to Make a Photo Look Fake

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The aim of this proposal is to obtain new knowledge of protein structure and function in the initiation of blood coagulation and fibrinolysis by two separate approaches. First, an attempt will be made to obtain protein structures by means of X-ray crystallography. This technique has been used successfully to obtain the structures of the first two chains of human plasminogen activator inhibitor 1, which is the first and most potent inhibitor of urokinase-type plasminogen activator. The relationship of the carboxyl-terminal sequences of the protein to its specific biological activity will be further examined. In order to test the accuracy of the crystal structure, structure-function studies will be carried out on mutant plasminogen activator inhibitors and urokinase-type plasminogen activator. The second approach involves the continuation of studies on the mechanism of fibrinogen activation in the presence of an active thrombin-thrombomodulin complex. We have purified a cell-derived protein, named Protein S-A, which markedly enhances activation of fibrinogen by thrombin-thrombomodulin. These studies will be extended to investigate the relationship between Protein S-A and plasminogen activator inhibitor, particularly the portion of the inhibitor which is N-terminal to the catalytic active site. The effect of thrombin-thrombomodulin on the structure and function of Protein S-A and plasminogen activator inhibitor will be further examined. Binding studies with radioactive thrombin and thrombomodulin will be carried out in order to correlate any structural changes induced by thrombin with binding to the thrombomodulin molecule. These studies will be extended to include affinity chromatography with radioactive thrombomodulin. In addition, the binding of mutant thrombin and mutant thrombomodulin will be examined. Two mutant forms of thrombomodulin will be constructed to test the importance of the NH2-terminal and COOH-terminal domains in function. These studies will be extended to include the production of a mutant form of thrombomodulin in which the Arg108 residue, which is found only in the human thrombomodulin molecule, will be replaced by His. A mutant thrombin, consisting of Arg75 replaced by His will also be constructed.Q:

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