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Cells of uncertain lineage (CUC) responses to Nodal signalling in the sea urchin embryo. The specification and maintenance of pattern during the formation of the primary germ layer in the sea urchin embryo requires the combined signals from both the vegetal and animal blastomeres. In particular, signalling from the vegetal pole has a crucial role in mesoderm pattern formation, whereas the role of signalling from the animal pole is less clear. Recent work has shown that the localisation of the Nodal receptor, Crumbs3, to a small number of cells, the ‘initiating cells’, within the vegetal plate correlates with the formation of ectodermal structures, and the maintenance of ectodermal fates. We now report an analysis of the characteristics of the ‘initiating cells’ and the neighbouring cells in the vegetal plate, and the effects of inhibitors of the Nodal signalling pathway. Our results suggest that the initiating cells respond to the localisation of Crumbs3 to the vegetal pole and contain transcripts of the gene at the onset of zygotic gene activation. In response to the Nodal signalling inhibitor, Wntless, the initiating cells become more widely spaced within the vegetal plate and the nearby cells appear to convert to a less responsive cell type. This corresponds to a reduction in the expression of the early zygotic gene, Ceac. We suggest a model for the initiation of signalling by the pre-zygotic expression of Crumbs3 in the vegetal plate and the response of the cells to the subsequent signalling from the vegetal pole.}, 225 (2000). T. Lee, K. Nishijima, and M. Okawa, Prog. Theor. Phys. **70**, 474 (1983). T. Lee and Y. Pang, Int. J. Mod. Phys. **A9**, 1369 (1994). D. M. Eliezer and R. J. Szabo, Phys. Lett. **B460**, 136 (1999). M. Wakamatsu, Eur. J. Phys. **25**, 659 (2004). M. V. Chizhov and J. I. Kapusta, Phys. Rev. **D71**, 065008 (2005); M. A. Shifman, Int. J. Mod. Phys.



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Deliciously healthy Chocolate Raspberry Cookies. These delicious 1-2-3 Cookies are simple to make! Use your food processor to blend flour, sugar and eggs then fold in your raspberry and chocolate chips. This Chocolate Raspberry Cookie recipe is so easy that the children can help with it! Let’s Connect! Current Hostess Code: Sign Up For Blog Updates, Recipes, & More! Enter Email Address Comments or Questions? All material on this site is copyright and cannot be republished without written permission. You may link to this site or link to content within this site, but please give credit to Simply Recipes (www.simplyrecipes.com) and send an email to rds(at)simplyrecipes(dot)com with a link to the specific content you are referring. Thank you!package io.vivo package edge import org.scalatest.{FlatSpec, Matchers} class LeaderCacheSpec extends FlatSpec with Matchers { “LeaderCache” should “produce the correct cached value, even when the return type of the function is String” in { val cachedValue = LeaderCache.getCachedValue(() => “Bar”) cachedValue.must_== “Bar” } “LeaderCache” should “produce the correct cached value, when it is an array of strings” in { val cachedValue = LeaderCache.getCachedValue(Array(“Bar”, “Baz”)) cachedValue.must_== “Bar” } } How To Refinance Your Mortgage I think that I have mentioned this before, but I’m doing what I can to pay off my student loans and I have a $20,000 balance. It is very slow going, but I’m making progress. In today’s economy, a lot of people are looking to switch lenders. Let me first explain why. There are many reasons to refinance your home loan. You may want to refinance if you want to: Lower interest rates, Reduce your mortgage payments, or Shorten the length of your loan. Let’s look at each of these in turn. Lower interest rates This is the most common reason to refinance your mortgage. The interest rates on your home



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